Duke University RESEARCH TECHNICIAN II - Human Vaccine Institute in Durham, North Carolina

99909BR

Auto req ID:

99909BR

Duke Entity:

MEDICAL CENTER

Job Code:

951 RESEARCH TECHNICIAN II

Job Description:

Research Technician II – Denny Lab

Responsibilities:

  • Perform assays assessing virus-specific immune responses against HIV

  • Process and isolate mononuclear cells from human and nonhuman primate blood and other tissues and conduct subsequent steps involved in the phenotypic evaluation of cells by flow cytometry, including cellular staining techniques and data acquisition, analysis, and maintenance.

  • Perform ordering responsibilities for the laboratory, including taking inventory, restocking, submitting orders, managing samples, and coordinating with other lab members regarding their specific assay needs.

  • Perform cell culture, virus, and reagent preparation for immunologic and virologic assays.

  • Perform antibody binding and neutralization assays for various laboratory projects.

  • Perform other laboratory or data management duties, including laboratory maintenance tasks, as requested by the Principal Investigator or senior lab staff.

  • Collect information and data through observation, experimentation and various other methods, to include operating highly technical laboratory instruments and equipment.

  • Prepare and process specimens for complex immunological and virological procedures, including but not limited to PBMC Processing, Flow Cytometry, Cell Culture, Real Time PCR, ELISA, Western Blot, and devise experimental techniques for testing and analysis as directed by laboratory manager.

  • Perform other duties as assigned.

Education, Skills, & Experience Requirements

  • Bachelor’s degree in biology or a related field

  • Laboratory experience is preferred

  • Must be detail-oriented, well organized and approach procedures with critical thinking of the technical aspects with strong communication skills and ability to work in an interactive team environment.

Location:

Durham

Exempt/Non-Exempt:

Non-exempt

Requisition Number:

401410136

Position Title:

RESEARCH TECHNICIAN II - Human Vaccine Institute

Shift:

First/Day

Job Family Level:

08

Full Time / Part Time:

FULL TIME

Regular / Temporary:

Regular

Department Name:

Duke Human Vaccine Institute - Admin

Minimum Qualifications:

Duke University is an Affirmative Action/Equal Opportunity Employercommitted to providing employment opportunity without regard to anindividual's age, color, disability, gender, gender expression, genderidentity, genetic information, national origin, race, religion, sex,sexual orientation, or veteran status.

Duke aspires to create a community built on collaboration, innovation,creativity, and belonging. Our collective success depends on the robustexchange of ideas—an exchange that is best when the rich diversity ofour perspectives, backgrounds, and experiences flourishes. To achievethis exchange, it is essential that all members of the community feelsecure and welcome, that the contributions of all individuals arerespected, and that all voices are heard. All members of our communityhave a responsibility to uphold these values.

Essential Physical Job Functions:Certain jobs at Duke University and Duke University Health System mayinclude essential job functions that require specific physical and/ormental abilities. Additional information and provision for requests forreasonable accommodation will be provided by each hiring department.

Education

Work requires a bachelor's degree in botany, biology, zoology or otherscience related scientific field.

Experience

None required above education/training requirement.OR AN EQUIVALENT COMBINATION OF RELEVANT EDUCATION AND/OR EXPERIENCE